Table 3.

Principle PBP mutants and the number of FACS iterations required to isolate cell shape suppressors

Expt no.a	Strain	PBPs deleted	No. of FACS iterations	Suppressor isolatedb
1	CS315	4, 5, 7	15	+ (ALM10)
2	CS315	4, 5, 7	4	+ (LCM1)
3	CS315	4, 5, 7	6	+
4	CS315	4, 5, 7	5	+
5	CS446	4, 5, 6, 7	4	+ (LCM2)
6	CS448	4, 5, 7, AmpH	7	−
7	CS449	4, 5, 7, AmpC	4	+ (LCM3)
8	CS456	1A, 4, 5, 7	6	−
9	CS534	4, 5, 7, AmpC, AmpH	9	−
10	CS545	1A, 4, 5, 6, 7	9	−
11	CS703	1A, 4, 5, 6, 7, AmpC, AmpH	4	−
12	CS703	1A, 4, 5, 6, 7, AmpC, AmpH	15	−

^aEach experiment is independent experiment. The least restrictive sorting gate was used in experiment 1. All other experiments were performed with the more circumscribed and more efficient sorting gates described in the text.

^b+, shape suppressors were generated; −, shape suppressors were not generated. Strain names for suppressor mutants discussed in the text are given in parentheses.