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K. kingae PilC1 and PilC2 contain calcium-binding sites. Calcium competition binding assays using Oregon green were performed with WT, D930A, and D930K recombinant PilC1739–1047-MBP (A); WT, D1444A, and D1444K recombinant PilC2868–1502 (B); and WT, D1125A, and D1125 recombinant PilC2868–1502 (C). Binding curves were modeled to one-site competition or linear line. Error bars represent standard errors of the means.
