Fig 5.

The PilC1 and PilC2 Ca-binding sites influence twitching motility. K. kingae strain KK03 and derivatives were assessed for twitching motility using a modified agar plate stab assay. Twitching-zone diameters were measured in triplicate, and averages were calculated from three independent experiments. Error bars represent standard errors of the means. Statistical analysis was performed using ANOVA with Bonferroni's posttest to compare twitching-motility zones between the mutants and the parental strains as noted in the text. Asterisks indicate statistically significant (P < 0.05) differences between the mutants and the relevant parental strain.