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Analysis of proline content in C. difficile cells. Extracts from C. difficile wild-type (JIR8094), LB-CD4 (prdA::ermB), LB-CD12 (grdA::ermB), and LB-CD8 (prdR::ermB) strains harvested after growth to mid-exponential phase in TY medium were assayed for free proline using a published procedure (48), and the results were expressed relative to those for the wild-type strain. Results represent the means and standard deviations of three biological replicates.
