Figure 3.

Long-term effect of mifepristone on tumor cell lines of the ovary (A), breast (B), nervous system (C), and prostate (D, E). Cells were seeded at a density appropriate for each cell line, allowed to adhere for 24 h, and then exposed to concentrations of mifepristone (MF) specific for each cell line for 72 h. Thereafter, triplicate wells were harvested by trypsinization and counted by microcytometry. Remaining wells were returned to vehicle treatment and monitored for 5 days, during which time triplicate wells were harvested and counted every 24 h. Growth expressed as number of cells per well are shown for each cell line (A-D). Data points represent the mean ± s.e.m of 3 independent experiments completed in triplicate. Viability of LNCaP cells was also determined by microcytometric analysis (E).