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. 2013 Feb 1;8(2):e55131. doi: 10.1371/journal.pone.0055131

Figure 1. Halloween gene expression during development and in adult Tribolium.

Figure 1

The relative mRNA distribution of the Halloween genes during development and in adults was measured by qPCR. Expression was normalized to rpS3 mRNA levels. The step in the ecdysteroid biosynthetic pathway catalyzed by each enzyme is shown on the right. Numbers on the x-axis indicate the day of each stage. E; embryo, F; females, O; ovaries, M; males, R; reproductive systems of males, 7dC; 7-dehydrocholesterol, 2,22,25-dE; 2,22,25-trideoxyecdysone (ketodiol), 2,22-dE; 2,22-dideoxyecdysone (ketotriol), 2-dE; 2-deoxyecdysone, 20E; 20-hydroxyecdysone. Note that the Black Box is believed to include multiple uncharacterized reactions converting 7dC to the ketodiol.