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. 2013 Feb 1;8(2):e55131. doi: 10.1371/journal.pone.0055131

Figure 2. Knock down of phm and spo delays development and reduces ecdysteroid levels in Tribolium.

Figure 2

Number of larvae that have undergone a molt after knock down of phm (phm-RNAi) and spo (spo-RNAi) expression 5 days (A) and 9 days (B) after injection with dsRNA. GFP dsRNA (GFP-RNAi) was used as a negative control. (C) Ecdysteroid levels in spo-RNAi and phm-RNAi larvae are reduced compared to the GFP control and to spot-RNAi 5 days after injection with dsRNA. Hemolymph ecdysteroid levels were below the limit of RIA detection (about 10 pg) in the hemolymph of spo-RNAi and phm-RNAi animals. L-L; larval-larval molt, L-P; larval-pupal molt.