Skip to main content
. 2013 Feb 1;8(2):e52408. doi: 10.1371/journal.pone.0052408

Figure 3. After 24 hours of treatment with IFNβ, inducible and regular subunits co-exist in the 20S particles.

Figure 3

(A). Size exclusion chromatography. Size exclusion chromatography of extracts prepared from IFNβ treated (top 3 blots) and untreated (bottom 2 blots) MIN6 cells was performed as described in Methods and the indicated gel filtration fractions (GF# 5–29, lanes 1–13) were analyzed by Western blot next to the indicated amounts of purified mouse His-β5 and His-β5i proteins (lanes 14–19). (B). Models with β5 and β5i subunits in a single 20S particle (left), and with 20S structure in which one of the two β5 subunits is marked in color, with C-terminus in red (right). (C). Immuno-precipitation. MIN6 extracts analyzed in A were subjected to immuno-precipitation (IP) with the indicated antibodies or control (ctr.) IgG from a pre-immune rabbit. The immunoprecipitates were analyzed by Western blot, as indicated.