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. 2013 Feb 1;8(2):e55361. doi: 10.1371/journal.pone.0055361

Figure 3. siRNA-mediated knockdown of TopBP1 and Claspin suppressed heat-induced Chk1 Ser345 phosphorylation and enhanced heat cytotoxicity.

Figure 3

A. Western blot. HeLa cells were transfected with siRNA for GFP, TopBP1#1 or TopBP1#2 and cultured at 42.5°C for 60 minutes. RI: relative intensity compared to the sample of siGFP and 42.5°C for 60 minutes. B. Clonogenic survival. HeLa cells were transfected with siRNA for GFP or TopBP1#1 and cultured at 42.5°C for the indicated time. C. Western blot. HeLa cells were transfected with siRNA for GFP or Claspin and cultured at 42.5°C for 60 minutes. RI: relative intensity compared to the sample of siGFP and 42.5°C for 60 minutes. D. Clonogenic survival. HeLa cells were transfected with siRNA for GFP or Claspin and cultured at 42.5°C for the indicated time.