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. 2013 Feb 1;8(2):e55361. doi: 10.1371/journal.pone.0055361

Figure 4. ATM-deficiency inhibited heat-induced Chk2 Thr68 phosphorylation and enhanced heat cytotoxicity.

Figure 4

A. Western blot. HeLa cells were cultured at 42.5°C for 60 minutes in the presence or absence of the ATM inhibitor KU55933 (ATMi). RI: relative intensity compared to the sample of ATMi (−) and 42.5°C for 60 minutes. B. Clonogenic survival. HeLa cells were cultured at 42.5°C for the indicated time in the presence or absence of ATMi. C. Clonogenic survival. Wild-type and Atm-deficient DT40 cells (WT or atm) were cultured at 45°C for the indicated time. D. Apoptosis. WT and atm DT40 cells were cultured at 45°C for 60 minutes and at 39.5°C for 60 minutes, and the increase in the number of early apoptotic cells induced by these treatments is shown. *p = 0.0131 (Student's t test). E. Western blot. HeLa cells transfected with siRNA for GFP or ATR (in the presence or absence of ATMi) were cultured at 42.5°C for 60 minutes. RI: relative intensity compared to the sample of siGFP and 42.5°C for 60 minutes. F. Clonogenic survival. HeLa cells transfected with siRNA for GFP or ATR (in the presence or absence of ATMi) were cultured at 42.5°C for the indicated time.