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. 2013 Feb 1;8(2):e55533. doi: 10.1371/journal.pone.0055533

Figure 4. Gene transfer of Ad5-PK vectors is CAR-independent.

Figure 4

(A) Fluorescence micrographs of CAR-negative CHO and human CAR (hCAR)-positive CHO-hCAR cell lines imaged 24 hours post-infection with Ad5GFP1-PK (300 v.p./cell), a vector that is isogenic to Ad5Luc1-PK except for the EGFP reporter gene. (B) Luciferase activities in CAR-negative CHO and CAR-positive CHO-hCAR cells following infection with Ad5Luc1 (open columns) or Ad5Luc1-PK (filled columns). Luciferase activities were determined 24 hours post-infection and reported in relative light units, RLU. Each column is the average of 3 independent replicates using 300 v.p./cell. Error bars indicate standard deviation. (C) Luciferase activities in CHO-hCAR cells following infection with Ad5Luc1 (open columns) or Ad5Luc1-PK (filled columns) in the presence of increasing concentrations of recombinant HAdV-5 fiber knob protein to competitively inhibit CAR-mediated cell binding of Ad vectors. Luciferase activities were determined 24 hours post-infection and reported in relative light units, RLU. Each column is the average of 4 independent replicates using 100 v.p./cell. Error bars indicate standard deviation. # indicates that p<0.0002 versus unblocked Ad5Luc1 control using the Student's t-test.