Table 1. Bacterial strains and plasmids used in this study.
Strains | Genotype | Source |
CFT073 | Wild-type Uropathogenic E. coli | H. L. Mobley [33] |
SM3010 | ΔuvrY::cam | CFT073 |
SM3011 | ΔcsrA::cam | CFT073 |
SM3013 | ΔuvrY::cam/p-uvrY | SM3010 |
SM3014 | ΔcsrA::cam/p-csrA | SM3011 |
MG1655 | Wild-type K-12 λ- rph-1 | D. J. Jin [34] |
AM1002 | ΔuvrY::cam | MG1655 |
AM1005 | ΔuvrY::cam/p-uvrY | AM1002 |
TR1-5MG1655 | ΔcsrA::kan | T. Romeo [36] |
AM1006 | ΔcsrA::kan/p-csrA | TR1-5 MG1655 |
Plasmids | ||
pBR322 | Cloning Vector | Invitrogen |
pSM2 | pBR322 containing uvrY gene; Apr | [29] |
pSM7 | pBR322 containing csrA gene; Apr | [29] |
pBB2-1 | fimA-lacZYA on pPR274 | William R. Schwan [40] |
pWS124-17 | fimA-lacZYA locked on on pPP2-6 | William R. Schwan [40] |