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. 2013 Feb 1;8(2):e55296. doi: 10.1371/journal.pone.0055296

Figure 3. Expression of Ihh, Ptch1, Gli1, Runx2, Runx3 and Sox9.

Figure 3

(A and A’) Ihh expressed in prehypertrophic chondrocytes. (B and B’) The Ihh expression pattern was not altered when hedgehog signaling one day of 5E1 injection at E12.5. The Ihh-expressing regions in second and third phalanges were connected following the injection of 5E1, but not after injecting PBS. (C and C’, E and E’) Ptch1 and Gli1 were expressed in the mesenchymal cells surrounding the prehypertrophic chondrocytes. (D and D’, F and F’) Ptch1 and Gli1 were down-regulated due to blocking the activity of the IHH protein. (G and G’, I and I’) Runx2 and Runx3 were expressed in mesenchymal cells, similar to Ptch1 and Gli1. (H and H’, J and J’) Runx2 and Runx3 were down-regulated when 5E1 was transferred. (K–L’) Sox9 expression did not differ between the PBS- and 5E1-treated groups. p1 (green box), condensation of phalange 1; p2/3 (red and yellow box), unseparated primordium of phalanges 2 and 3; p2/3* (orange box), connecting region of Ihh expression; m, metacarpals; A, anterior; D, distal part of the limb; (A–L, scale bar = 1 mm. A’–L’; longitudinal section view, scale bar = 100 µm.).