Figure 3.

Expression and sequence of novel Ame-mir-0002. Embryos are shown anterior to the left, dorsal side up. (A) Sequence of the predicted mir-0002 precursor, aligned below are the sequence reads and associated read counts. Highlighted in red is the sequence with the highest read count. Bracket-notation of secondary structure is beneath the precursor sequence. (B) The predicted mir-0002 hairpin structure, with the most abundant read sequence highlighted in red. (C) Genomic location of the predicted pre-mir-0002 miRNA is within the vesicular glutamate transporter gene on chromosome 11. (D) Expression of pri-Ame-mir-0002 during embryogenesis. At 24 hours following oviposition (stage 4), expression of pri-Ame-mir0002 is detected through the ectoderm. (E) By stage 6 (30 hours), staining for pri-Ame-mir-0002 is lost from the regions of the ectoderm were the extraembryonic membranes are being patterning, the anterior-dorsal and ventral side of the embryo. (F) At later stages, pri-Ame-mir-0002 RNA is detected in the neuroectoderm.