Figure 4.

Expression and sequence of novel Ame-mir-0004. The embryo is orientated anterior to the left, dorsal side up. (A) Sequence of the predicted mir-0004 precursor with the sequence reads aligned below and corresponding read counts. Bracket-notation of secondary structure is shown. Highlighted in red is the sequence with the highest read counts. (B) The predicted mir-0004 hairpin structure, with the most abundant read sequence highlighted in red which is likely to correspond to the mature miRNA. (C) Intergenic location of the pre-mir-0004 on chromosome 15. (D) Expression of pri-Ame-mir-0004 during early embryogenesis. Nuclear expression is detected in the ectoderm at both the anterior and posterior poles. (E) Expression at stage 4 was detected throughout the embryo but enriched at each pole.