Figure 5.

Expression and sequence of novel Ame-mir-0007. Embryos are positioned anterior to the left, dorsal side up. (A) Sequence of the mir-0007 precursor with aligned sequence reads and read counts. Highlighted in red is the sequence with the highest read counts. Bracket-notation of secondary structure is shown below the precursor. Mature miRNA and mirRNA* sequences are shown surrounded by asterisks. (B) The predicted mir-0007 hairpin structure, the most abundant read sequence highlighted in red. (C) Genomic location of the pre-mir-0007; mir-0007 is located between the predicted genes LOC726303 and LOC410888 on chromosome 2. (D) Expression during embryogenesis at stage 4. (E) Expression of pri-mir0007 at stage 9 is though the posterior of the extraembryonic membrane.