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. 2013 Jan 16;7(1):011808. doi: 10.1063/1.4774307

Figure 5.

Figure 5

Batch-mode DEP-FFF elution profiles for two mixtures of cancer cells with normal peripheral blood mononuclear cells: (A) Normal PBMNs + MDA-MB-231 breast tumor cells run at a DEP operating frequency of 65 kHz; (B) Mormal PBMNs + HL-60 promyelocytic leukemia cells run at 80 kHz. Different colors denote cells of differing size ranges. Note that the elution times for the cancer cells have a weak dependency on cell size because their DEP crossover frequencies dominate their elution behaviors. The scale at top shows the height at which the cells travelled through the DEP chamber as deduced from their elution times in the Poiseiulle flow profile. The cancer cells were tracked by prelabelling them with green-fluorescent calcein-AM viable cell stain (L-3224, Molecular Probes, Life Technologies).