Figure 2. Overexpression of Odc1 can promote the ES cell state in the absence of LIF. (A) Alkaline phosphatase staining of ESCs grown in the absence of LIF with and without Odc1 overexpression for 5 d. ESCs and cells transfected with the empty vector are shown as a control. (B) Real-time PCR showing the maintenance of high levels of Oct4, Sox2 and Klf4 when Odc1 is overexpressed in cells differentiated in the absence of LIF for 5 d. ESCs and cells transfected with the empty vector are shown as a control. (C) Western blot analysis showing high levels of OCT4 and SOX2 on overexpression of Odc1. GAPDH is shown as a loading control.