Figure 4. Binding of p53 to TDG promoter region. TE-13 cells were transfected with increasing amounts p53 expression vector (p53wt-pcDNA3) as indicated. Cross-linked chromatin was immunoprecipited with anti-p53 antibody CM1 (p53Ab) or control antibody (Ctrl) and analyzed by polymerase chain reaction (PCR) using primers specific for TDG promoter region encompassing p53RE2/3. MW, molecular weight markers; input, non-precipitated cross-linked chromatin.