Figure 3. Depletion of PRMT1v2 induces apoptosis. Representative flow cytometric analyses of propidium iodide (PI)-stained MCF7 cells following control or si/sh-PRMT1v2 transfection at 24, 48 and 72 h (A). Percentage of subG1 population for MCF7 (B) and T47D (C) cells. Data are the mean ± standard error of five (for MCF7) and four (for T47D) independent experiments (*p < 0.05). Representative flow cytometric analyses of Annexin V and PI co-staining of MCF7 and T47D cells following control or si/sh-PRMT1v2 transfection for 24 h (D). Percentages in each quadrant represent the mean ± standard error of three independent experiments (*p < 0.05). Total protein lysates were collected from MCF7 and T47D cells that were mock and control transfected for 24 h or transfected with si/sh-PRMT1v2 for 6, 12, 18 and 24 h. Western blot analysis for the expression of PARP shows the appearance of its cleavage product in MCF7 (E) and T47D (F) cells transfected with si/sh-PRMT1v2. GAPDH was used as a loading control.