Figure 4. Depletion of PRMT1v2 inhibits breast cancer cell motility and invasion. MDA-MB-231 cells depleted of PRMT1v2 were assessed for effects on motility and invasion. Western analysis for PRMT1v2 and PRMT1v1 following 24 h depletion (A). GAPDH serves as a loading control. Following 24 h of PRMT1v2 depletion, cells replated at equal numbers into Transwell chambers and incubated for an additional 24 h. Motility was analyzed using Transwell chambers without a Matrigel (- Matrigel). Invasion was analyzed using Transwell chambers containing a Matrigel layer (+ Matrigel). Representative images of cells that have passed through the Transwell chamber -/+ Matrigel at 20X magnification (B). Cell numbers that passed through the Transwell chambers in the absence (C: motile cells/field) or presence (D: invasive cells/field) of Matrigel were determined. Data represents the mean ± standard error of three independent experiments (*p < 0.05 comparing to control).