Figure 7. Overexpression of PRMT1v2 in MCF7 cells alters their morphology. Representative phase contrast and GFP fluorescence images of MCF7 cell stably expressing GFP, PRMT1v1-GFP or PRMT1v2-GFP proteins (A, arrowsheads indicate the presence of filopodia and lamellipodia, magnifications are indicated to the left). Fluorescence images of actin filament staining using FITC-conjugated phalloidin in MCF7 cells stably expressing GFP, PRMT1v1-GFP and PRMT1v2-GFP (B, arrowsheads indicate the presence of filopodia and lamellipodia, magnifications are indicated to the left). A colony dispersion assay was used to quantify the effects on colony formation. Representative images of compact, loose and scattered colonies (C). Distribution of compact, loose and scattered colonies in MCF7 cells stably expressing GFP, PRMT1v1-GFP or PRMT1v2-GFP (D). Ten thousand cells were plated and grown as described in ref. 29. Approximately 70 colonies were counted for each cell line per experiment. Colonies were scored by three independent investigators. Data are the mean ± standard error from four independent experiments (*p < 0.05, comparing compact colony numbers).