Figure 10. Enhanced autophagy in αSNAP-depleted epithelial cells does not depend on BNIP1. (A and B) SK-CO15 cells were subjected to sequential transfections with one of the following siRNA pairs: control-control, control-BNIP1, control-αSNAP and BNIP1-αSNAP. Expression of targeted proteins and autophagic markers was determined by immunoblotting at 48 h after the second transfection. #p < 0.01 compared with control siRNA-transfected cells. (C and D) HeLa-GFP-LC3 cells were transfected with either control or BNIP1-specific siRNAs and accumulation of autophagosomes was monitored by fluorescence spectroscopy. Scale bar, 20 µm; *p < 0.001 compared with control siRNA-transfected cells. (E) HeLa-GFP-LC3 cells subjected to αSNAP depletion were immunolabeled for BNIP1 (red) at 72 h post-transfection. Scale bar, 10 µm.