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Figure 3. Loss of αSNAP decreases expression of mTOR and its downstream effector, 4E-BP1. SK-CO15 cells were transfected with either control or αSNAP-specific siRNAs. Expression and phosphorylation of mTOR and its downstream effectors p70 S6 kinase and 4E-BP1 in total-cell lysates was determined at different times post-transfection.
