Figure 1. Western blot results showing that the Mps1 weak allele ald^B4–2 retains approximately 16.4% of the endogenous Mps1 protein. (A) Western blot results showing samples prepared from the third instar larvae brains for w⁶⁷, ald^B4–2, gfp-cdc20; ald^B4–2 and gfp-mad2; ald^B4–2 lines. A small amount of the Mps1 endogenous protein in samples from ald^B4–2, gfp-cdc20; ald^B4–2 and gfp-mad2; ald^B4–2 lines can be still detected. Expression of GFP-Cdc20 (panel 2) and GFP-Mad2 (panel 3) fusion proteins has been confirmed in the relevant ald^B4–2 genetic background as indicated. The actin bands are used as a loading control. All the antibodies were used in 1:500 dilutions. The western blot bands were imaged using an Odyssey machine. (B) Quantification results showing the western blot band intensities for Mps1 in relation to the above samples after normalization with the intensity of the relevant actin band. The data are presented as a percentage of the Mps1 in the wild-type (w⁶⁷) sample. The intensity for each sample was quantified from three independent western blots using TINA software.