Figure 4. The level of kinetochore recruitment of Cdc20 is not affected by reduction of the Mps1 in ald^B4–2 mutant. (A) Spinning disk confocal time-lapse images show GFP-Cdc20 localization patterns in third instar larvae neuroblasts from wild-type (gfp-cdc20; ald⁺, top panel) or Mps1 mutant (gfp-cdc20; ald^B4–2, bottom panel). Arrows indicate kinetochore-associated GFP-Cdc20 signals. Arrowheads indicate the prophase nucleus just before NEB (nuclear envelope breakdown, Image at 00:00 time point) or the early interphase nucleus (Image at 26:00 time point) where GFP-Cdc20 was excluded from the nucleus. Bar = 2μm. (B) GFP-Cdc20 fluorescent intensities at prometaphase kinetochores (selected regions a and c) were quantified and compared after subtraction of the relevant cytoplasmic backgrounds (selected regions b and d). Spinning disk confocal images displaying the peak levels of the GFP-Cdc20 at the prometaphase kinetochores were taken from either the wild-type (gfp-cdc20; ald⁺, left) or the Mps1 mutant (gfp-cdc20; ald^B4–2, right). Bar = 3 μm. (C) The graph shows the quantification results. There is no significant different between the highest intensities of the GFP-Cdc20 recruited at prometaphase kinetochores in Mps1 mutant (gfp-cdc20; ald^B4–2) and in the wild-type (gfp-cdc20; ald⁺) neuroblasts. Twenty individual neuroblast images from each fly line were used for this quantification. Time, minutes, seconds.