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. 2013 Feb 1;8(2):e55372. doi: 10.1371/journal.pone.0055372

Figure 6. STS-induced apoptosis was prevented in 661W cells in the presence of α-Crystallins.

Figure 6

661W cells transduced with the recombinant lentiviruses overexpressing αA-crystallin (pWPI_αA), αB-crystallin (pWPI_αB) or the empty lentivirus (pWPI) were treated with 100 nM STS for 16 h. (A) STS-triggered apoptosis was inhibited in the presence of α-crystallins, as reflected by TUNEL assay using TMR-dUTP. (B) STS-induced caspase activation was decreased in 661W cells overexpressing αA- and αB-crystallins, as measured by colorimetric Caspase-3/-7 assay. (* p<0.005 by t-test for pWPI versus pWPIαA). Data are the mean ± SE of four independent experiments.