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. 2013 Feb 1;8(2):e55320. doi: 10.1371/journal.pone.0055320

Figure 1. Western blot analysis of Rv1430 full-length protein and PE-PPE domain by anti-His antibody.

Figure 1

Lane 1, Uninduced E. coli BL21 (DE3) CodonPlus-RIL transformed with Rv1430 construct; Lane 2, Induced sample- Rv1430 (59.3 kDa); Lane 3, Protein Marker; Lane 4, lysate of E. coli Rosetta gami transformed with an empty vector; Lane 5, Uninduced Rv1430 PE-PPE domain sample; Lane 6, Induced sample- Rv1430 PE-PPE domain (25.8 kDa). Lane 7, Induced sample-Ser199Ala mutated Rv1430 PE-PPE domain (25.8 kDa).