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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Gene Expr Patterns. 2012 Oct 4;13(1-2):21–29. doi: 10.1016/j.gep.2012.09.001

Fig 4.

Fig 4

Mesoderm organ systems immunostained for SPL protein with positive cytoplasmic signal, counterstained with hematoxylin. E8: A. Amnion (arrows) and yolk sac (YS) with moderate positive signal. Scale bar 800 um. B. Blood island (arrowhead) of yolk sac with positive endothelial lining and positive fetal precursor red blood cells. Scale bar 200 um. C. Amnion (arrow) parietal (arrowhead) and visceral (*) layers of yolk sac with positive signal. Scale bar 200 um. E12: D. Moderately positive signal in myocardium (m), negative in red blood cells (arrowhead). Scale bar 800 um. E15: E. Mild positive signal in myocardium (m), negative in red blood cells (arrowhead). Scale bar 800 um. E18: F. Negative signal in myocardium (m) and red blood cells (arrowhead). Scale bar 800 um. E12: G. Positive signal in primordial thymus (derivative of third brachial arch) (arrow). Scale bar 400 um. E15: H. Minimal scattered positive signal in lymphocytes (arrow) of thymus, but had mild to strong patchy positive signal in thymic epithelial (reticular) cytoplasm (arrowheads), which are derived from endoderm. Scale bar 400 um. E18: I. Negative signal in lymphocytes (arrow) of thymus, but scattered mild to strong positive signal in thymic epithelial (reticular) cytoplasm (arrowheads), which are derived from endoderm. Scale bar 400 um.

HHS Vulnerability Disclosure