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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Gene Expr Patterns. 2012 Oct 4;13(1-2):21–29. doi: 10.1016/j.gep.2012.09.001

Fig 5.

Fig 5

Endoderm organ systems immunostained for SPL protein with positive cytoplasmic signal, counterstained with hematoxylin. E12: A. Lung bud with positive signal in cytoplasm of primordial lung epithelium (arrows). Scale bar 400 um. B. Intestinal epithelium with positive cytoplasmic signal (arrow). Scale bar 400 um. C. Higher magnification of B showing signal near apical portion of epithelial cells. Scale bar 200 um. E15: D. Lungs with moderate to strong cytoplasmic signal throughout the tissue. Scale bar 400 um. E. Developing intestinal tract with minimal to mild cytoplasmic staining in future submucosa, muscularis mucosa and serosa (arrows). Scale bar 800 um. F. Higher magnification of E reveals mild cytoplasmic staining in mucosal cells of intestinal tract (arrowhead). Scale bar 200 um. E18: G. Absence of immunohistochemical signal in lung. Scale bar 400 um. H. Minimal cytoplasmic signal in developing submucosa, muscularis mucosa and serosa (arrows). Scale bar 400 um. I. Mild cytoplasmic signal in apical cytoplasm of enterocytes (arrowheads). Scale bar 200 um.

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