. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: J Proteome Res. 2013 Jan 2;12(2):743–752. doi: 10.1021/pr300805f

Table 1.

Neuropeptides observed in ESI mass spectra of each sample preparation experiment^a

			Experiment 1		Experiment 2		Experiment 3		Experiment 4

Family	[M⁺ H]⁺	Sequence	acMeOH	Stabilizor T1	DMF	Stabilizor T1	DMF	Boiling	Boiling	Stabilizor T1
AST-A	781.39	DPYAFGLa				⁺				⁺
	854.40	DGPYSFGLa	⁺	⁺	⁺	⁺
	883.43	SNPYSFGLa								⁺
	^b893.42	SGHYNFGLa	⁺						⁺	⁺
	897.46	ARGYDFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	909.49	ARPYSFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	911.47	ARAYDFGLa							⁺	⁺
	928.45	SSGQYAFGLa					⁺	⁺		⁺
	937.53	PRVYSFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	939.50	TRPYSFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	962.51	APQPYAFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	^b986.48	RNMYSFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	998.49	TSPQYSFGLa			⁺	⁺
	1004.48	FSGTYNFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1021.51	THPTYSFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1097.60	AGLSALYSFGLa		⁺	⁺
	1167.58	AAGLQNYDFGLa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	2051.03	GSGQYAYGLGKKAGQYSFGLa	⁺	⁺	⁺		⁺	⁺	⁺

AST-B	1031.51	AWSNLQGAWa	⁺	⁺	⁺	⁺		⁺	⁺	⁺
	1061.55	AGWSSLKGAWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1107.52	AGWSSMRGAWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1123.51	AGWSSM(O)RGAWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	^b1165.55	NWNKFQGSWa	⁺	⁺	⁺		⁺	⁺	⁺
	1182.57	TSWGKFQGSWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1209.58	TGWNKFQGSWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1220.58	SGDWSSLRGAWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1222.58	GNWNKFQGSWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1252.59	NNWSKFQGSWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1253.57	NDWSKFGQSWa	⁺	⁺	⁺	⁺	⁺
	1293.63	STNWSSLRSAWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1380.64	NNNWTKFQGSWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1470.70	VPNDWAHFRGSWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1586.84	MFAPLAWPKGGARWa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

AST-C	^b1899.83	pQIRYHQcYFNPIScF	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

CCAP	956.38	PFCNAFTGCa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

FLP	^b695.40	NFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	^b851.50	RNFLRFa			⁺
	^b916.52	THPFLRFa	⁺	⁺	⁺		⁺		⁺	⁺
	^b925.45	DDNFLRFa	⁺	⁺	⁺	⁺		⁺
	926.52	SKNYLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	965.54	NRNFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	966.53	DRNFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1022.56	GNRNFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1104.61	GAHKNYLRFa	⁺		⁺	⁺	⁺		⁺	⁺
	1124.63	GLSRNYLRFa	⁺	⁺	⁺	⁺	⁺		⁺	⁺
	^b1132.60	NVGSHGFLRFa	⁺	⁺		⁺	⁺		⁺
	1146.61	GYSKNYLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1147.65	APQRNFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1158.62	YGNRSFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

Myosuppressin	1125.57	pQDLDHVFLR			⁺	⁺	⁺	⁺	⁺
	1271.65	pQDLDHVFLRFa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1288.68	QDLDHVFLRFa	⁺	⁺	⁺	⁺	⁺		⁺

Orcomyotropin	1186.52	FDAFTTGFGHS	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

Orcokinin	1228.56	NFDEIDRSSFa	⁺	⁺	^#	⁺
	1256.55	NFDEIDRSGFG	⁺	⁺
	1270.57	NFDEIDRSGFA	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1403.62	NFDEIDRSGFGF	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1433.63	NFDEIDRSSFGF	^#	⁺	^#	⁺	^#	^#		⁺
	1474.66	NFDEIDRSGFGFA	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1502.69	NFDEIDRSGFGFV	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1504.67	NFDEIDRSSFGFA	^#	⁺	^#	⁺	^#	^#	^#	⁺
	1532.70	NFDEIDRSSFGFV	^#	⁺	^#	⁺	^#	^#	^#	⁺
	1547.68	NFDEIDRSSFGFN	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

Others	844.48	HLGSLYRa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

Proctolin	649.37	RYLPT	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

RYamide	784.41	FVGGSRYa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	832.41	FYANRYa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	959.47	SGFYAPRYa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	976.46	SGFYANRYa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺
	1027.54	SRFVGGSRYa					⁺	⁺	⁺	⁺
	1030.47	pEGFYSQRYa	⁺	⁺	^#	⁺	⁺	⁺	^#	⁺
	1114.58	SSRFVGGSRYa	⁺	⁺	⁺	⁺	⁺	⁺	⁺	⁺

Total Peptides			60	62	59	61	64	61	57	58
Unique Peptides			2	1	5	2	6	2	6	4

AST, allatostatin; CCAP, crustacean cardioactive peptide; CPRP, crustacean hyperglycemic hormone precursor related peptide; FLP, FMRFamide like peptide. Amidation indicated by lowercase a. Pyroglutamation indicated by lowercase p. Disulfide bond indicated by lowercase c. Methionine oxidation indicated by M(O).

indicates peptide had BPI > 1000, but no MS/MS event triggered

⁺

indicates that peptide was observed in ESI spectra

indicates that there are additional peaks at [M+2H]⁺² that mask neuropeptide signal