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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Mamm Genome. 2012 Oct 25;24(1-2):30–43. doi: 10.1007/s00335-012-9436-9

Figure 2. Western blot analysis confirming epitope-tagged RCAN1 expression in brain, thymus, and heart, with expression proportional to copy number of the BACTg.

Figure 2

A, Total proteins were prepared from BAC-Tg and wild type mouse tissues and blotted with anti-HA antibody to recognize the epitope-tagged human RCAN1 protein. The HA antibody detects the expression of two isoforms of RCAN1 in brain, thymus and heart. The Coomasie blue stained gel is shown as a loading control. B, Western blot probed with a RCAN1-specific antibody that recognizes RCAN1 proteins of both human and mouse. Comparison of the three BAC-Tg mouse lines shows copy-number dependent RCAN1 protein expression.