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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Mamm Genome. 2012 Oct 25;24(1-2):30–43. doi: 10.1007/s00335-012-9436-9

Figure 4. The protein encoded by the LOC73419 gene interacts substoichiometrically with RCAN1.

Figure 4

A, Plasmids encoding HA-LOC73419 and Flag-RCAN1 were co-expressed in 293 cells. After IP of Flag-RCAN1, HA- LOC73419 is detected. The input lysate for the IP (4% of total) has been loaded in the first lane; comparison with the IP lane shows that the interaction of these two proteins in 293 cells is sub-stoichiometric. B, HA-LOC73419 and Flag-RCAN1 plasmids were co-transfected into Neuro-2a cells, with an unrelated control expression plasmid, producing Flag-USP21, serving as a negative control. IP was performed as in (A). Ha-LOC73419 is more efficiently pulled down by IP of Flag-RCAN1. The negative control Flag-USP21 shows no binding to Ha-LOC73419. The first lane shows 1% input; comparison with the results in (A) suggests that HA-LOC73419 interacts more efficiently with Flag-RCAN1 in Neuro-2a cells than in 293 cells.