Fig.2.

Sequence analysis of heterologously expressed YFV-17D RNA by three-step 5′-RACE. (A) Sequences of cDNA clones from pSV40–YF17D transfected cells were aligned with the predicted circularized 5′ cDNA as expected progeny from the SV40 origin/promoter fusion to the YFV-17D cDNA in pSV40–YF17D (second line). The predicted major transcriptional start site for the SV40 promoter/origin (+1, top line) according to Refs. [7,8] is shown. The EcoRV recognition site derived from the 5′ end of ODN 1 is underlined. Dots represent the consensus with the predicted sequence, and dashes represent nucleotide deletions.