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. 2013 Jan 15;4:1353. doi: 10.1038/ncomms2358

Figure 1. RNase P in vitro cleavage assays performed with Arabidopsis recombinant PRORP1 and variants of mitochondrial tRNACys precursors.

Figure 1

+ and − indicate the absence and presence of PRORP proteins in the reactions. WT is the wild-type tRNACys, ΔAC the tRNA without the anticodon domain, and ΔDAC without both anticodon and D domains. G18A, for example, shows a tRNA where the guanosine at position 18 was mutated into an adenine. 1CG72 is a tRNA where the G-C base pair at positions 1 and 72 was swapped to a C-G. Δ3′-shows a tRNA precursor without 3′-trailer sequence and 3′-CCA, the precursor with a mature 3′-end containing a CCA. P stands for tRNA precursors, M the 5′-mature products and L the cleaved 5′-leader fragments. The molecular weights of markers are given in nucleotides. PRORP cleavage products were quantified with ImageGauge (Fujifilm). Values were normalized so that 100 corresponds to the cleavage efficiency observed for wild-type tRNACys precursor. Cleavage efficiencies are given below the respective panels together with s.d.’s for three representative experiments.