Table 1. Inductively coupled plasma mass spectrometry identifies zinc in association with PRORP.
PRORP1 | Zn66 (p.p.b.) | 2σ | Stoichiometry |
---|---|---|---|
WT | 29.49 | 1.53 | 1 |
C344A | 23.82 | 0.46 | 0.8 |
C347A | 20.89 | 0.24 | 0.7 |
H548A | 11.80 | 0.50 | 0.4 |
C565A | 7.44 | 0.21 | 0.2 |
DD474-475AA | 28.52 | 1.49 | 1 |
Buffer | 0.44 | 0.12 | 0 |
Measurements were performed on wild-type PRORP1 (WT), as well as on proteins with point mutations applied to positions predicted to form the zinc-binding pocket. 2σ indicates the s.e. in four replicate measurements.