Figure 2. Col IV is synthesized in the basal ER.
(A–E) mRNAs encoding the Col IV α-chains (A) Cg25C and (B) Vkg, and the Col IV biosynthetic enzymes (C) Plod and (D) PH4αEFB are all enriched in the basal cytoplasm. α-Spectrin marks the apical and lateral epithelial surfaces. (E) PH4αEFB-RNAi causes Vkg-GFP to accumulate in the basal ER. The dashed line marks the apical surface. Experiments performed at stages 7–8. Scale bars are 10 μm. See also Figure S2.