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Immunization with adenovirus formulations containing chitosan does not augment Treg populations. (a) Balb/c mice were immunized with Ad5-PSA and (b) C57BL/6 with Ad5-OVA formulated with CpG and/or chitosan. CD4+Foxp3+ Treg cells were detected 14 days later by flow cytometry. Means ± SD from 2 mice/immunization group are shown.
