Table 1.
Cell and tissue staining protocols.
| Primary antibody | Secondary antibody | |||||
|---|---|---|---|---|---|---|
| Antigen | Producer | Antigen retrieval | Dilution | Fluorochrome | Producer | Dilution |
| OCT4 | ABCAM | TRITONX-100 0.1% | 1 : 200 | A488 | Molecular probes | 1 : 400 |
| NANOG | ABCAM | TRITONX-100 0.1% | 1 : 400 | A488 | Molecular probes | 1 : 400 |
| SOX2 | ABCAM | TRITONX-100 0.1% | 1 : 200 | A488 | Molecular probes | 1 : 400 |
| eGFP | ABCAM | TRITONX-100 0.1% | 1 : 80 | A488 A555 |
Molecular probes | 1 : 400 1 : 600 |
| MCM5 | ABCAM ABCAM |
TRITONX-100 0.1% | 1 : 80 1 : 100 |
A555 A488 |
Molecular probes | 1 : 600 1 : 400 |
| eGFP MMP2 |
ABCAM ABCAM |
TRITONX-100 0.1% | 1 : 80 1 : 80 |
A488 A555 |
Molecular probes | 1 : 400 1 : 600 |
| eGFP ASA |
ABCAM Sigma |
TRITONX-100 0.1% | 1 : 80 1 : 100 |
A488 TRITC [IgM] |
Molecular probes Jackson ImmunoResearch |
1 : 400 1 : 100 |
| eGFP CD146 |
ABCAM ABCAM |
TRITONX-100 0.1% | 1 : 80 1 : 20 |
A488 A555 |
Molecular probes | 1 : 400 1 : 600 |
| eGFP SMA |
ABCAM DAKO |
TRITONX-100 0.1% | 1 : 80 1 : 40 |
A555 A488 |
Molecular probes | 1 : 400 1 : 600 |
| Laminin ASA |
DAKO Sigma |
TRITONX-100 0.1% | 1 : 50 1 : 100 |
A488 Cy5 [IgM] A555 |
Molecular probes Jackson ImmunoResearch |
1 : 400 1 : 100 1 : 600 |