Table 1.

Antimutagenic potential of extracts from fractions RA, RB, and RC obtained from lipidic extract of octopus (average^a TA100 and TA98 revertants/plate, with S9).

Extract fractionc	Fraction dilutionc
	1 × 100	1 × 10−1	1 × 10−2	1 × 10−3
TA98
RA (R f = 0.0–0.29)	170 ± 13	296 ± 24	267 ± 13	333 ± 18
RB (R f = 0.29–0.69)	117 ± 10	271 ± 18	338 ± 27	350 ± 31
RC (R f = 0.69–1.0)	215 ± 7	387 ± 23	299 ± 21	453 ± 33
TA100
RA (R f = 0.0–0.29)	71 ± 16	203 ± 12	567 ± 35	621 ± 53
RB (R f = 0.29–0.69)	127 ± 3	361 ± 39	534 ± 37	567 ± 24
RC (R f = 0.69–1.0)	185 ± 15	565 ± 43	523 ± 48	536 ± 30

^aAFB₁ (used as positive control) tested at 100, 500, and 1000 ng/plate induced 195 ± 23, 495 ± 29, and 676 ± 55 and 200 ± 16, 595 ± 43, and 745 ± 24 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 55 ± 7 and 113 ± 19 for TA98 and TA100, respectively.

^bValues are means of three replicates ± SEM.

^cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB₁/plate.