Table 3.
Anti-mutagenic potential of AFB1 a -spiked fractions obtained from thin layer chromatography fractionation of fraction RB2 (R f = 0.78–1.00) from TLC 2 (averageb TA100 and TA98 revertants/plate, with S9).
| Extract fractionc | Fraction dilutionc | |||
|---|---|---|---|---|
| 1 × 100 | 1 × 10−1 | 1 × 10−2 | 1 × 10−3 | |
| TA98 | ||||
| RB21 (R f = 0.00–0.47) | 49 ± 9 | 73 ± 4 | 257 ± 20 | 325 ± 24 |
| RB22 (R f = 0.47–0.79) | 71 ± 3 | 141 ± 20 | 219 ± 19 | 218 ± 28 |
| RB23 (R f = 0.79–1.00) | 18 ± 2 | 175 ± 13 | 236 ± 18 | 265 ± 21 |
|
| ||||
| TA100 | ||||
| RB21 (R f = 0.00–0.47) | 175 ± 30 | 198 ± 18 | 220 ± 15 | 275 ± 71 |
| RB22 (R f = 0.47–0.79) | 292 ± 12 | 455 ± 31 | 843 ± 27 | 883 ± 12 |
| RB23 (R f = 0.79–1.00) | 320 ± 25 | 695 ± 40 | 567 ± 44 | 1687 ± 70 |
aAFB1 (used as positive control) tested at 500 ng/plate induced 1006 ± 83 and 1689 ± 78 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 55 ± 3 and 188 ± 14 for TA98 and TA100, respectively.
bValues are means of three replicates ± SEM.
cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB1 plate.