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. Author manuscript; available in PMC: 2013 Jun 14.
Published in final edited form as: Mol Cell. 2012 Nov 8;48(5):760–770. doi: 10.1016/j.molcel.2012.10.002

Figure 5. Gene regulation by non-canonical sites.

Figure 5

(a) Gene expression differences assessed by microarray for canonical targets and non-canonical targets without seed matches in the 3′UTR in miR-155-sufficient and -deficient T cells. (b) Repression of 3′UTRs with non-canonical target sites by miR-155 assessed in luciferase reporter assays. Percent repression is the reduction in luciferase activity observed upon miR-155 overexpression relative to the luciferase activity observed with control miRNA overexpression. Socs1, a canonical target, the Socs1 miR-155 site mutant and Tgm2, were used as a positive and negative controls, respectively. (c) Repression of luciferase reporter expression for constructs with and without mutations in the predicted non-canonical sites. Mutations are shown in Figure 3b. (d) Gene expression differences from microarray data (Mu et al., 2009) of canonical targets and non-canonical targets that lacked miR-17~92 seed matches anywhere in the 3′UTR in wild type and miR-17~92 cluster deficient transformed B cells. Predicted non-canonical targets contain AGO binding sites with a single mismatch from miR-17~92 seed matches and an exact match to a 3′ motif. In (b) and (c) mean ± SEM is plotted, *, p<0.05; **, p<0.01; ****, p<10-4.