Figure 3.

Membrane specificity of the LPS effect on Caco-2 permeability. A: Effect of LPS (0.3 ng/mL) on Caco-2 TER. The mean TER for control (Cont) Caco-2 monolayers was 475 ± 12 Ω • cm². B: Effect of LPS (0.3 ng/mL) on Caco-2 inulin flux. LPS (0.3 ng/mL) was added to either apical (AP), basolateral (BL), or combined apical and basolateral compartments (Both) for the 5-day experimental period. C: Effect of increasing concentrations of LPS (0, 0.1, 0.3, 1, 20, 1000, and 50,000 ng/mL) on Caco-2 TER. The mean TER for control Caco-2 monolayers was 454 ± 19 Ω • cm². D: Effect of increasing concentrations of LPS (0, 0.1, 0.3, 1, 20, 1000, and 50,000 ng/mL) on Caco-2 inulin flux. LPS was added to the apical compartment at each 24 hours treatment period for 5 days. *P < 0.0001 versus control.