Figure 4.
Effect of LPS on Caco-2 cell apoptosis and proliferation. A: Effect of increasing concentrations of LPS on Caco-2 cell apoptosis was assessed by flow cytometry. LPS concentration of 1 mg/mL was used as a positive control of apoptosis.23 There were a total of 10,000 events for each sample. B: Effect of LPS on caspase-3 expression assessed by Western blot analysis. C: Effect of LPS on Caco-2 cell proliferation as assessed by mitochondrial dehydrogenase conversion of tetrazolium salt WST-1 to formazan. *P < 0.0001 versus control.