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Effect of LPS on CD14 expression in filter-grown Caco-2 monolayers. A: Time-course effect of LPS on CD14 expression. B: Effect of siRNA CD14 transfection on Caco-2 TER. The mean TER for control Caco-2 monolayers was 482 ± 15 Ω • cm2. C: Effect of siRNA CD14 transfection on Caco-2 inulin flux. D: Effect of siRNA TLR-4 transfection on expression of CD14 in Caco-2 cells. Scr, scrambled. *P < 0.001 versus control; **P < 0.001 versus LPS treatment.
