Figure 2.

HLA-G transcriptional activity in 21 glioblastoma biopsy samples (G1 to G21). Results of real-time RT-PCR performed twice (each in triplicate) are presented, with two distinct sticks (mean of triplicates) for each sample, except G7 (only one experiment). Results are compared to the HLA-G mRNA expression in HLA-G–positive choriocarcinoma JEG-3 cells (assigned a value of 1) and HLA-G–negative cell lines melanoma M8 and glioblastoma U251MG. Glioblastoma specimens studied in IHC are underlined. Plus (+) and minus (−) signs indicate positive and negative HLA-G protein expression (IHC), respectively.