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. 2013 Feb;182(2):540–552. doi: 10.1016/j.ajpath.2012.10.021

Figure 4.

Figure 4

Induction of HLA-G gene transcription in HLA-G–negative glioma cells after treatments with 100 μmol/L 5-aza-dC and 5-aza-dC/IFN-γ (500 U/mL). Real-time RT-PCRs (performed in triplicate for each experiment) targeting all HLA-G mRNA forms are shown for each glioma cell line (U251MG, U138MG, and D247MG), either untreated (UT) or treated with 5-aza-dC alone, 5-aza-dC and IFN-γ combined, and IFN-γ alone, for 5 days. The y axis indicates amounts of HLA-G mRNA levels relative to mRNA expression in HLA-G–positive JEG-3 choriocarcinoma cells (assigned a value of 1). Data are presented as mean ± SEM for at least three independent experiments. Statistical analysis was performed using U-tests; *P < 0.05 and **P < 0.01.