Figure 7.

APM components and β2-m up-regulation in U251MG glioma cell line upon 5-aza-dC and IFN-γ treatments. Representative Western blots are presented (at least two independent experiments). Protein extracts (50 μg) are from U251MG cells either untreated (UT) or treated with 5-aza-dC alone, 5-aza-dC/IFN-γ combined, and IFN-γ alone. Results obtained from the same gel are framed. Quantitative variations are indicated; a value of 1 is assigned to the amount of APM component in untreated cells or to the amount of HLA-G expression in cells treated with 5-aza-dC alone.