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Gdn-induced changes in the Dns–heme distance distributions [P(rDA)] for Dns c552 variants at pH 3.0, extracted from TR fitting of fluorescence decay curves (Fig. S3): Gdn 0 M (black), 3 M (red), 3.5 M (yellow), 4 M (green), 4.5 M (cyan), 5 M (blue), and 7 M (magenta). The area of each bar reflects the probability amplitude over the corresponding distance range. Group I and group II variants display three- and two-state folding transitions, respectively.
