Fig. 4.

Caspase-1 and ASC are dispensable for the allergic response to bee venom. (A) WT (n = 5), Casp1^−/− (n = 5), and Asc^−/− (n = 5) mice were immunized s.c. with Human Serum Albumin (HSA) (100 μg per mouse) or bee venom (100 μg per ) in PBS on days 0 and 21. Serum IgE production was measured by ELISA on days 0, 14, and 28. (B) WT (n = 4) and Casp1^−/− (n = 4) mice were immunized with endotoxin-free OVA (100 μg per mouse) and melittin (100 μg per mouse) on days 0 and 21, and OVA-specific IgE in the serum was measured on day 28.